New Life Hiking Spa in Vermont has been helping clients lose pounds and maintain a healthy weight for the last 40 years. Their non-clinical approach uses mountains, clean air and lush greenery instead of an indoor gym, plus wholesome natural food instead of a diet. To assist with a healthy lifestyle following a weight loss retreat New Life offers nutrition and wellness workshops and individual counseling.   
Weight loss tips # 1: Drink at least eight, 8-ounce glasses of water per day. Beverages with little or no calories, caffeine or sodium, including herbal tea, are best. Avoid regular soft drinks and soups with lots of sodium. If you are eating plenty of water-rich foods such as fruits, vegetables and low-sodium soups, you can get half of your water requirements from foods, according to a 1998 NAS Food and Nutrition Board report.
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We’ve been clear on the benefits of wild salmon, but those pink creatures are quite literally not the only fish in the sea. Generally speaking, fish provide one of the best sources of fatty acids known as omega-3s, which will help fend off waist-widening inflammation and are an excellent source of high-quality, lean protein. This allows them to help you maintain muscle mass, thus reducing excess fat accumulation. Some of our favorite healthy seafood include mussels, Atlantic mackerel, and bluefish, but be sure to educate yourself on the ocean dwellers with this list of Every Popular Fish—Ranked for Nutritional Benefits!
Wondering why it's so hard to lose that muffin top? There are so many environmental and personal factors that contribute to our putting on pounds and it's not enough to enroll in the latest fad diet or jog for half an hour every day to ensure weight loss. To successfully lose weight and keep it off you have to look at everything from your nutrition and fitness to your sleep habits and stress management. This may seem like a lot but Mountain Trek program can work for you.
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Moving your muscles against resistance helps them grow and get stronger. Having more muscle mass also means that you burn more overall calories. Resistance training has profound effects on your bones and joints, and helps to prevent osteoporosis (loss in bone mineral density), sarcopenia (loss of muscle mass), and lower-back pain, assuming you use proper exercise form.6
The next time you’re making a salad, why not throw some watercress in there? The green veggie is an excellent source of folate, which has been shown to stimulate weight loss. In fact, a study in the British Journal of Nutrition found that those with the highest folate levels lose about 8.5 times more weight when dieting than those with the lowest levels of folate. What’s more? A separate study in the British Journal of Cancer found that higher dietary folate intake reduces the breast cancer risk. In addition to watercress, other good sources of folate include spinach, asparagus, and papaya.
This diet consists of forcing yourself to only eat lean, unfried meat, water, eggs and vegetables, with no fat and just a tiny bit of bread or fruit – hardly a laugh a minute! Apparently the combination of foods is supposed to interact chemically to burn fat. We’ll admit that this diet may be a quick fix for that upcoming wedding or holiday but an extreme diet plan like the chemical diet is not nutritionally balanced and does not contain enough calories for a healthy lifestyle.
Calcium and vitamin C team up well to boost metabolism, and broccoli is just one of several healthy foods that contains both nutrients. What sets broccoli apart from the others, however, is that the green veggie also contains kind of fiber that’s been shown to increase the digestion, absorption and storage of food, also known as the thermic effect of food (TEF). A revved up metabolism combined with an increased TEF is a match made in weight loss heaven, so consider incorporating broccoli into a tasty stir-fry, or serving it as its own side dish.
It’s insane what some will do to have a slight edge in a fight. The biggest example that comes to mind is Anthony Johnson. The dude would walk around at like ~230 lbs and fight in the 170 lb weight class. He would rag-doll guys because of the huge size advantage. Of course, he always had trouble making weight, but it’s still a huge advantage if you can do it properly.
Apparently there’s a special ingredient in grapefruit that, when eaten with a form of protein, triggers a fat-burning process and therefore results in weight loss. So, the idea is to start each meal with half a grapefruit, eat lots of protein and drink plenty of water and black coffee. While this diet may help you lose a few pounds, any weight lost will pile straight back on once you return to your normal diet. Such a drastic reduction in your intake of calories can also result in dizziness and an upset stomach, plus it’s so very boring!
Whilst we are not exclusively a weight loss retreat for women, over 70% of the guests attending our program are women of a variety of ages. Many of their stories are unique but all share some major commonalities; their busy lives have driven them into a state of non-activity; poor eating habits, confused about health and nutrition, eating processed foods that are quick and easily consumed on the fly. Combine that with the number of hormones (like Leptin and Cortisol) running rampant throughout the body and it’s no wonder our bodies are enslaved by cravings and have stopped metabolizing effectively; becoming fat-storing machines.
Some popular beliefs attached to weight loss have been shown to either have less effect on weight loss as commonly believed or are actively unhealthy. According to Harvard Health, the idea of metabolism being the "key to weight" is "part truth and part myth" as while metabolism does affect weight loss, external forces such as diet and exercise have an equal effect.[43] They also commented that the idea of changing one's rate of metabolism is under debate.[43] Diet plans in fitness magazines are also often believed to be effective, but may actually be harmful by limiting the daily intake of important calories and nutrients which can be detrimental depending on the person and are even capable of driving individuals away from weight loss.[44]
At your conveniently located One Body Weight Loss & Wellness Center in the Boat Club Road area of Fort Worth, TX, our medically supervised weight loss program supports behavior modification and nutritional education to help our patients succeed. No appointments are necessary, though we recommend them so you don’t have to wait for an opening. Our staff of medical professionals has been specially trained in weight loss, nutrition and healthy living. Be sure to stop in and find out how we can help you achieve your weight goals even if every other diet you’ve tried has failed. We’ll show you the one that works!
While some people respond well to counting calories or similar restrictive methods, others respond better to having more freedom in planning their weight-loss programs. Being free to simply avoid fried foods or cut back on refined carbs can set them up for success. So, don’t get too discouraged if a diet that worked for somebody else doesn’t work for you. And don’t beat yourself up if a diet proves too restrictive for you to stick with. Ultimately, a diet is only right for you if it’s one you can stick with over time.
21. Keep it simple. "I take a minimalist approach to nutrition: My diet consists of lean protein (chicken breast, egg whites, ground turkey), complex carbs (quinoa, sweet potatoes, oatmeal), healthy fats (coconut oil, almonds, avocados), and leafy green veggies. I eat as clean as I can—locally-grown vegetables, organic when possible, and minimally-processed everything."
There's a reason you've been hearing so much about cutting meat out of your diet lately. It's not just great for your body, but also a quick way to shed some extra pounds. "Consider swapping a few meat-centric meals each week for ones centered around vegetarian proteins — or give a full-fledged vegetarian diet a try if that's of interest to you," Gorin says. "Research shows eating a vegetarian diet may boost and speed up weight loss, resulting in a loss of up to 10 pounds." Gorin recommends topping a salad or filling a veggie taco with vegetarian protein sources like pulses — which are beans, chickpeas, lentils, and dried peas — to give your weight loss a boost. One study found eating ¾ cup of pulses daily led to a loss of close to a pound over about six weeks, versus people not eating pulses daily.
A calorie isn’t always a calorie. Eating 100 calories of high fructose corn syrup, for example, can have a different effect on your body than eating 100 calories of broccoli. The trick for sustained weight loss is to ditch the foods that are packed with calories but don’t make you feel full (like candy) and replace them with foods that fill you up without being loaded with calories (like vegetables).
More than half of the attendees at In:spa are repeat visitors thanks to a realistic, holistic approach to wellness. Cal Reiet, a fantastically smart 15-bedroom manor house, provides the perfect base. Programmes are a mix of combined activities (HIIT workouts, a 10km coastal hike, yoga) and more tailored, emotionally nourishing sessions (nutrition advice and massages). It's not particularly flexible, but relinquishing decision-making for a week is hugely relaxing, as is exercising in such lovely surroundings. At the heart of the experience is the food, a menu of mainly vegetarian dishes such as seed bread slathered in almond butter and avocado, turmeric and pea salad. It's a programme that will keep making you feel better, even long after you have left.
Why a top-pick? Many weight loss retreats develop their programs around a specific exercise schedule and predetermined nutritional goals. At Kripalu, participants spend more time delving into why they eat and what they can do to make healthier choices. Dieters don't go home with a specific meal plan to follow, but they have the opportunity to connect with others so that they can continue to build on the foundation that they establish at Kripalu.

One Body helps you understand the critical question of how and why you may have gained weight, and then clearly lays out a plan to help you lose weight long term. You will be given a low carb, high protein diet and regimen, that will help shed pounds week after week. Many One Body patients lose up to 15-20 pounds the first month* and up to 2-5 pounds per week thereafter.
One Body helps you understand the critical question of how and why you may have gained weight, and then clearly lays out a plan to help you lose weight long term. You will be given a low carb, high protein diet and regimen, that will help shed pounds week after week. Many One Body patients lose up to 15-20 pounds the first month* and up to 2-5 pounds per week thereafter.
One study from the University of Adelaide in Australia suggests you may lose more weight when you work out towards the end of your menstrual cycle, as opposed to right when a new one begins. That’s because the hormones estrogen and progesterone tell your body to use fat as an energy source. "Women burned about 30 percent more fat for the two weeks following ovulation to about two days before menstruation," study author Leanne Redman says.
Listen up: Skipping meals will not make you lose weight faster. If a hectic day makes a sit-down meal impossible, stash an energy bar or a piece of fruit in your car or tote, keep snacks in your office desk drawer, and make a point of getting up to grab a nosh — anything that will keep you from going hungry! Going long periods of time without food does double-duty harm on our healthy eating efforts by both slowing down your metabolism, and priming you for another binge later in the day. (Think: You've skipped breakfast and lunch, so you're ready to takedown a whole turkey by dinner!) Make it your mission to eat three meals and two snacks every day, and don't wait longer than three to four hours without eating. Set a "snack alarm" on your phone if needed.

Apparently there’s a special ingredient in grapefruit that, when eaten with a form of protein, triggers a fat-burning process and therefore results in weight loss. So, the idea is to start each meal with half a grapefruit, eat lots of protein and drink plenty of water and black coffee. While this diet may help you lose a few pounds, any weight lost will pile straight back on once you return to your normal diet. Such a drastic reduction in your intake of calories can also result in dizziness and an upset stomach, plus it’s so very boring!


For those new to the Mayr cure, this efficacious diet plan developed by Dr Franz Xaver Mayr is based on the principle that giving an overworked digestive system a break allows other parts of the body to repair themselves. Such are its remarkable effects that the method has reached legendary status when it comes to tackling fatigue, arthritis, migraine and sleep problems. The food is healthy, modern Mayr at its best - no sugar, no dairy, no alcohol. Many people come here to lose weight, others to press pause, still more for an annual health MOT. The vast medical department offers everything from traditional massages and intravenous drips to bone-density measurements and hi-tech oxygen therapy used by astronauts. The diagnostics are mind-bogglingly impressive - blood tests, mineral tests, cardiovascular tests, intolerance tests - all of which dictate your treatments, as well as lay bare the real state of your health.
When it’s doing its job properly, inflammation in the body helps fight off infection. But in people who are overweight or obese, inflammation can get out of control, which increases insulin resistance as well as your risk for heart disease, cancer, arthritis, and even Alzheimer’s disease. “Excess body fat, especially when it’s located around the abdomen, is strongly linked to inflammation,” Dr. Webster says. Adipose fat tissue releases inflammation-causing molecules, but the markers of inflammation go down with even a 5 or 10 percent reduction in body weight, she says. Eat more of these 15 superfoods that can help you lose weight.
Even if you fill up on produce, lean proteins, and whole grains, according to British Journal of Nutrition findings, when you think about the quality of your diet, you’re likely forgetting about all the unhealthy food that also finds its way to your mouth. People tend to exaggerate the good foods they eat and underestimate the bad stuff, says study author, Kentaro Murakami, PhD of Japan’s University of Shiga Prefecture. While it’s not necessarily intentional, it’s likely one of the reasons why it’s so hard for people to lose weight. For example, you might grab a handful of candy at a co-worker’s desk or a sample at the mall and then forget about it altogether. Our advice: To get a more accurate overview of your diet, keep a detailed food journal on your phone—yes, that means you should include that food court sample, too. Whether you snap photos or keep a written log is totally up to you—both tactics will work. The more food records dieters kept over the course of 30 months, the more weight they lost, a study in the American Journal of Preventive Medicine found.
“The Fit Body Weight Loss Program is the ONLY thing that has ever worked for me. I have already lost 70 pounds in nine months and I have dropped 6 dress sizes. I still have 20 more pounds to lose, which I believe I will lose in the next month. I feel better than I’ve ever felt and I have so much more energy! This was the greatest investment into my health I have ever done!”
One Body helps you understand the critical question of how and why you may have gained weight, and then clearly lays out a plan to help you lose weight long term. You will be given a low carb, high protein diet and regimen, that will help shed pounds week after week. Many One Body patients lose up to 15-20 pounds the first month* and up to 2-5 pounds per week thereafter.
Cardio improves definition and burns the fat that covers your muscles, especially belly fat. Combining regular aerobic exercise with strength training will give you the slimming effect you've been going for. After all, toning without cardio is like building a house on a weak foundation. Blast calories with these 20-minute cardio workouts from celebrity trainer Jackie Warner.

The next time you’re making a salad, why not throw some watercress in there? The green veggie is an excellent source of folate, which has been shown to stimulate weight loss. In fact, a study in the British Journal of Nutrition found that those with the highest folate levels lose about 8.5 times more weight when dieting than those with the lowest levels of folate. What’s more? A separate study in the British Journal of Cancer found that higher dietary folate intake reduces the breast cancer risk. In addition to watercress, other good sources of folate include spinach, asparagus, and papaya.
© 2018 Condé Nast. All rights reserved. Use of and/or registration on any portion of this site constitutes acceptance of our User Agreement (updated 5/25/18) and  Privacy Policy and Cookie Statement  (updated 5/25/18). SELF may earn a portion of sales from products that are purchased through our site as part of our Affiliate Partnerships with retailers. Your California Privacy Rights.  The material on this site may not be reproduced, distributed, transmitted, cached or otherwise used, except with the prior written permission of Condé Nast. Ad Choices 
the best way for a woman to lose belly fat

At baseline, 6 months, and 24 months, fasting plasma glucose, insulin, total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and triglycerides were measured on the Synchron CX7 (Beckman Coulter), and hemoglobin A1C (HbA1c) was measured on a Synchron CX5 (Beckman Coulter); plasma leptin and soluble leptin receptor were measured by an ultrasensitive immunoassay (R&D Systems); and serum free triiodothyronine (T3), free thyroxine (T4), total T3, total T4, and thyroid stimulating hormone were measured using a competitive electrochemiluminescence immunoassay on the Roche E modular system (Roche Diagnostics), as previously described elsewhere [37]. The homeostatic model assessment of insulin resistance (HOMA-IR) value was calculated using the updated HOMA model (HOMA2) described by Levy et al. [42]. Adipose tissue was obtained from 103 participants at baseline and at 6 months. Gene expression was measured by direct hybridization using the Illumina HumanHT-12 v3 Expression BeadChip (Illumina) (details in S1 Text). 

Whatever the reason may be, weight loss is a common goal for many people. You don’t need to justify yourself, here. I get it. There are so many reasons to lose weight if you have weight to lose, that have nothing to do with fat shaming. Our bodies were not meant to be overweight, but with our current lifestyles, it seems to be an inevitable consequence!

Absolute Sanctuary Thailand fitness and weight management retreats range from 7-10-14 days,  and include meeting with a panel of medical experts for consultations and analysis, fitness assessments, nutritionally balanced spa cuisine from the resort’s Love Kitchen (providing whole foods, vegetarian and raw cuisine), nutritional supplements, group yoga and fitness classes,  cardio bootcamp and aquarobics, personalized fitness, herbal steam rooms and infrared sauna sessions, spa treatments and more.
You'll learn and experience Mindful Eating, a practice that helps you better understand why you choose to eat along with guided training and nutritional support. We have included acupuncture and spa therapies to promote your bodies natural ability to detoxify. When compared to other resorts, our Utah weight loss retreats include more individual testing and personalized attention – plus our special ingredient: adventure.
(CONFRONT YOUR FEARS) A friend reminded me of this recently and as he did I remembered a fear. It was fresh and still made my skin prickle with anticipation even though it started many years ago. It feels like yesterday and I’m sure you have those moments too, the ones that never left you even though you shoved them away for a long time. It starts with the 6th grade physical fitness tests. They were the worst things ever except for the talented few that got the bright blue patches. These tests traumatized me. Mostly, the timed mile. Even though we were children it was expected that we run it and everyone did- except me. There wasn't much kindness or compassion in the process and once I realized that, it was something that I dreaded, avoided and ultimately feared. It’s so easy to take ourselves out of even trying when there’s no encouragement to try. When your gym clothes don’t fit right and you’re afraid of the attention that coming in last place will bring you. Year after year this test happened and year after year I walked it & then cried, faked being sick and then ultimately stopped showing up. I’ve never run a mile without stopping and it’s always been on my list of things I want to do but didn’t know if I would. But I want to now. No matter how long it takes me and for no one else other than myself. And now, after being here I would rather try and fail than not try at all. Now’s the time to tackle the fears, all of them no matter how small or how big. So one day I started by asking my trainer if we could work on learning how to run in between strength training. My voice raised and I stumbled over my words and I felt self-conscious again like that chubby little 6th grader in too tight of gym shorts. I was nervous putting this out into the world. He said absolutely and then the next day we did. Just like that. He took me through some movements to work on my stride and made me feel like this was something that I could do. And step by step it was. Only 45 seconds this time, but 45 seconds closer to my goal and each step we take towards our goals away from our fears count. Not always the end result matters most, but the time it takes to jog there.
Make sure that everything you're eating is whole — as in nothing processed or packaged. Since salt is a preservative, these are the foods that are highest in sodium — something to keep in mind when planning your meals. Plan on making sure that all items you choose are fresh. That means filling up on fresh fruits and veggies, whole grains, low-fat dairy, and lean protein.
This Algarve hotel combines a seaside setting that's hot and close to home, with comprehensive wellness retreats. Sign up for a bootcamp and your first glimpse of the sand could be while running up and down the 149 steps of the wooden boardwalk that leads down to Falésia beach. Twice. There are also circuits - burpees on the lawn, tricep dips on the tennis courts, press-ups on a tyre by the family pool - and mountain bike rides through pine forests and along red-soil cliffs towards Vilamoura Marina. The other five programmes range from de-stress to detox, and include personalised meal plans, one-on-one fitness sessions and non-surgical cosmetic procedures like mesotherapy for targeted fat reduction. The vibe is friendly and laid-back - there's often live music in the courtyard, and when there is a full moon, a bar is set up on the sand and a reggae band plays late into the night.
“Fad diets and meal replacement shakes are not the answer to sustainable weight loss or better health. Sure, you can do something drastic to lose 20 pounds in a month, but chances are these actions aren’t sustainable. If you want to lose the weight and keep it off for good, target a weight loss of one to two pounds per week so you can truly see permanent, long-lasting results!” Kristen Carlucci Haase RD-N dished out in 22 Top Weight Loss Tips, According to Nutritionists.

Apparently there’s a special ingredient in grapefruit that, when eaten with a form of protein, triggers a fat-burning process and therefore results in weight loss. So, the idea is to start each meal with half a grapefruit, eat lots of protein and drink plenty of water and black coffee. While this diet may help you lose a few pounds, any weight lost will pile straight back on once you return to your normal diet. Such a drastic reduction in your intake of calories can also result in dizziness and an upset stomach, plus it’s so very boring!

© 2018 Condé Nast. All rights reserved. Use of and/or registration on any portion of this site constitutes acceptance of our User Agreement (updated 5/25/18) and  Privacy Policy and Cookie Statement  (updated 5/25/18). SELF may earn a portion of sales from products that are purchased through our site as part of our Affiliate Partnerships with retailers. Your California Privacy Rights.  The material on this site may not be reproduced, distributed, transmitted, cached or otherwise used, except with the prior written permission of Condé Nast. Ad Choices 

the best way for a woman to lose belly fat

On the raw food diet you can eat anything that is unprocessed and uncooked, so knock yourself out with your choice of organic fruit and veg, nuts, seeds, beans, seaweed and purified water. The creators of this diet are very strict about one thing, that only 75% of food must be heated over 116°C. Fancy living on a diet of only raw vegetables? No, neither do we!

In a 2-year diet-induced weight-loss trial (the POUNDS Lost trial), we measured plasma concentrations of PFASs at baseline in 621 overweight and obese men and women and collected information on changes in body weight, resting metabolic rate (RMR), and other metabolic parameters during weight loss and weight regain over the 2 years the participants were on the study diet.
Welcome to Whistler Fitness Vacations! We are an intensive weight loss immersion, exclusively for women of beginner fitness levels. Exclusively for those who outside of healthy weight range, our program is striving to be Canada’s leading luxury weight loss retreat. With weekly programs from May through September, each guest is lodged in private suites at the beautiful Westin Resort and Spa Whistler. Since 2008, we’ve run almost 400 retreats; helping women like you smash their goals, upgrade their health, and feel better about the body they’re in.
Spa Index Guide to Day Spas, Resorts, and Destination Spas. SpaIndex.com helps you find the best day spas, medical spas, hotels and resorts, weight loss retreats, weight loss spas, detox retreats, stop-smoking retreats and smoking cessation programs, luxury destination spas, wellness getaways, spa packages, DIY recipes, spa cuisine recipes, and the best spa lifestyle ideas and tips.

Dinner is similar to lunch but there are daily specials that we can choose from in addition to the set menu, followed by another thermal walk. At the end of the day, I always have a cup of my favorite cinnamon tea and then I focus on self-care, whether that's by taking a hot shower, a shopping trip, or enjoying a game night with friends. One of the best parts of coming here has been meeting all these amazing people going through the same journey I am. I didn't know anyone when I started and now I feel like I have a whole group of supportive friends.


It's a one-time investment you'll never regret. Here's why: Strength training builds lean muscle tissue, which burns more calories — at work or at rest — 24 hours a day, seven days a week. The more lean muscle you have, the faster you'll slim down. How do you start strength training? Try some push-ups or a few squats or lunges. Use your free weights to perform simple bicep curls or tricep pulls right in your home or office. Do these exercises three to four times per week, and you'll soon see a rapid improvement in your physique.
While ketchup and BBQ sauce are frequently used to help flavor beef, chicken, and the like, the tasty condiments are no friend to your waistline. Ketchup, for example, typically contains around 19 calories and 4 grams of belly-bloating sugar per tablespoon, and BBQ sauce is just as unhealthy, if not worse. To avoid consuming empty calories and unhealthy added sugar, have condiments such as mustard and sauerkraut on hand. While mustard has been linked to revving your metabolism, fermented sauerkraut will help balance the bacteria in your gut.
Interval training can also be a very effective weight-loss tool. It's pretty easy to incorporate into any fitness plan because it can be applied to a variety of settings, and different types of equipment can be used. Choose an activity that you can do at a high intensity (greater than 80 percent of your maximum heart rate) for 30-60 seconds, then follow it with 30-60 seconds of rest.

“Chia seeds aren’t just a pet, they’re a party in your mouth. I’m a huge fan of them because they’re chock-full of heart-healthy omega-3s, fiber, protein, and calcium,” Sarah Koszyk, MA, RD, founder of Family. Food. Fiesta. tells us in 26 Most Overlooked Ways to Lose Weight. “Chia seeds are easily absorbed by the body, so they’re very nourishing and satiating. Every day I add them to my breakfast smoothie or pair them with yogurt or cottage cheese along with some blueberries.”
Calcium and vitamin C team up well to boost metabolism, and broccoli is just one of several healthy foods that contains both nutrients. What sets broccoli apart from the others, however, is that the green veggie also contains kind of fiber that’s been shown to increase the digestion, absorption and storage of food, also known as the thermic effect of food (TEF). A revved up metabolism combined with an increased TEF is a match made in weight loss heaven, so consider incorporating broccoli into a tasty stir-fry, or serving it as its own side dish.
The iconic Red Rocks Amphitheatre in Colorado is known for its off-the-charts music scene, but it's the fitness offerings we're really interested in. Book the Red Rocks Recharge package at Hotel Teatro through summer 2016 and not only will you get a fresh-pressed green juice to fuel your mornings, but you'll also receive complimentary transportation to the theater to participate in the Yoga on the Rocks program. Want to raise your heart rate a little more? Join the Fitness on the Rocks program, where you'll incorporate the stairs into a sweaty, sweaty routine. A private car shuttles your exhausted (but proud) self back to your hotel where you can soak in a glorious recovery bath.
Plasma concentrations of PFOS, PFOA, perfluorononanoic acid (PFNA), perfluorohexanesulfonic acid (PFHxS), and perfluorodecanoic acid (PFDA) were measured at baseline only, using a sensitive and reliable method based on online solid phase extraction and liquid chromatography coupled to a triple quadropole mass spectrometer [41], with minor modifications. Due to the long elimination half-lives of the PFASs and incomplete samplings, we did not measure plasma PFAS levels during the trial. For all major PFASs, the concentrations were above the limit of detection (0.05 ng/ml), and the inter- and intra-assay coefficients of variation were <6.3% and <6.1%, respectively.

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