Harvie, M. N., Pegington, M., Mattson, M. P., Frystyk, J., Dillon, B., Evans, G., … Howell, A. (2011, May). The effects of intermittent or continuous energy restriction on weight loss and metabolic disease risk markers: A randomized trial in young overweight women. International Journal of Obesity (London), 35(5), 714–727. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017674/
Have you ever noticed children take forever to eat? That’s a good thing. A 2015 study found when people took 30 seconds in between bites, they stopped eating when they were no longer hungry, so they saved calories and pounds. This example of more chewing and less weight gain has been proven in several studies. We tend to consume more food than necessary as we age because emotion and stress can take over. To combat this, I suggest going back to childhood habits by using smaller plates and chewing longer. A smaller plate will make a smaller portion of food seem bigger. Focusing on slowing down your eating will help fill you up without filling you out.

Gabel, K., Hoddy, K. K., Haggerty, N., Song, J., Kroeger, C. M., Trepanowski, J. F., … Varady, K. A. (2018, June 15). Effects of 8-hour time restricted feeding on body weight and metabolic disease risk factors in obese adults: A pilot study. Nutrition and Healthy Aging, 4(4), 345–353. Retrieved from https://content.iospress.com/articles/nutrition-and-healthy-aging/nha170036


Intentional weight loss is the loss of total body mass as a result of efforts to improve fitness and health, or to change appearance through slimming. Weight loss in individuals who are overweight or obese can reduce health risks,[1] increase fitness,[2] and may delay the onset of diabetes.[1] It could reduce pain and increase movement in people with osteoarthritis of the knee.[2] Weight loss can lead to a reduction in hypertension (high blood pressure), however whether this reduces hypertension-related harm is unclear.[1][not in citation given]
Fighting constantly with your S.O.? It’s time to address your issues head-on. "Research has shown that cortisol, the hormone that's released during stressful activity, is linked to fat storage,” says Gina Guddet, couples counselor and co-author of Love Metabolism. “And poor communication between couples is the most common type of stress that you tend to experience."
If you've seen the TV show, you get the idea: Six weeks of healthy food and regular exercise is celebrated as a great start to a weight-loss journey – as well as a way prevent or reverse various diseases. Fair enough. Experts determined that the Biggest Loser Diet is very likely to help you shed pounds, thanks to calorie restriction and exercise. To reap the other benefits of weight loss, however, you have to stick with it – something that's a lot harder for average Joes than for TV stars-in-the-making.
Assume a prone position on the floor with the knees fully extended and the toes pointed down to the floor. Place hands on floor, palms down, about 2 to 3 inches wider than shoulder-width apart, with elbows pointed outward. Keeping your body in straight line and toes in contact with the floor, push against the floor with the hands to fully extend the elbows. Pause at the top and then lower to starting position.
Please check out Tribe of Mentors, my newest book, which shares short, tactical life advice from 100+ world-class performers. Many of the world's most famous entrepreneurs, athletes, investors, poker players, and artists are part of the book. The tips and strategies in Tribe of Mentors have already changed my life, and I hope the same for you. Click here for a sample chapter and full details. Roughly 90% of the guests have never appeared on my podcast.
The primary strength of the current study is that the cause of weight changes was well characterized. Unlike previous observational studies in which reasons for weight changes were usually unknown, this weight-loss trial applied energy restriction to induce the weight changes. Moreover, repeated measurements of body weight, RMR, thyroid hormones, leptin, and other metabolic biomarkers allowed documentation of longitudinal associations between PFAS exposures and changes in these parameters during the weight-loss and weight regain periods.
Body fat gets a bad rap, but your body needs it. There is an essential amount of fat that each body needs in order to maintain its natural functions. That amount will vary between body type, age, sex, physical activity level, and fitness goal. For the general population, the levels accepted as healthy are between 21-32 percent for women and 8-19 percent for men.1 Higher levels can lead to weight-related health risks such as Type 2 diabetes, certain types of cancer, and cardiovascular disease.
Lord Byron wasn’t only famous for his poems and politics, but also for popularising the vinegar diet in the 1820s. The idea is simple – drink plenty of vinegar, paired with one cup of tea and a raw egg. This combination made you vomit, causing you to lose your appetite, and weight. The legacy lives on in the claim that apple cider vinegar can make you lose weight.
Some popular beliefs attached to weight loss have been shown to either have less effect on weight loss as commonly believed or are actively unhealthy. According to Harvard Health, the idea of metabolism being the "key to weight" is "part truth and part myth" as while metabolism does affect weight loss, external forces such as diet and exercise have an equal effect.[43] They also commented that the idea of changing one's rate of metabolism is under debate.[43] Diet plans in fitness magazines are also often believed to be effective, but may actually be harmful by limiting the daily intake of important calories and nutrients which can be detrimental depending on the person and are even capable of driving individuals away from weight loss.[44]
Not like you needed another reason to fall in love, snuggle up with your sweetie, kiss or get it on. Harvard Medical School researchers found that all of those things can aid weight loss. How? Lovey-dovey feelings cause levels of the hormone oxytocin to increase, which in turn, decreases appetite. For even more weight loss hacks, check out these 20 Weight Loss Tricks You Haven’t Tried.
Cal-a-Vie bridges the gap between health resort and luxury spa.  Its comprehensive nutritional services include consultations with a registered dietician and tests with a biochemistry focus to identify problems arising from adrenal, thyroid or hormone imbalances, nutritional deficiencies, food sensitivities, and more. The fitness program includes more than 130 different classes for beginners to the already fit.
Studies show that eating breakfast plays a part in successful weight loss — almost 80 percent of people who successfully keep weight off chow down on this meal, according to a study published in Obesity Research. "Your metabolism slows as you sleep, and the process of digesting food revs it up again," explains Heller. Aim for a 300- to 400-calorie breakfast, such as a high-fiber cereal (another metabolism booster) with skim milk and fruit.
We have a wide array of fitness activities, pet free and pet friendly housing options, life-changing educational workshops, healthy meal plans, and optional pampering services such a massages and pedicures. You are sure to be rejuvenated and ready to implement lifelong changes when it is time to check-out of the spa. Join us for a fun fitness vacation at our adult weight-loss spa, nestled in the beautiful foothills of Tennessee; an award-winning fitness resort.
The next time you’re making a salad, why not throw some watercress in there? The green veggie is an excellent source of folate, which has been shown to stimulate weight loss. In fact, a study in the British Journal of Nutrition found that those with the highest folate levels lose about 8.5 times more weight when dieting than those with the lowest levels of folate. What’s more? A separate study in the British Journal of Cancer found that higher dietary folate intake reduces the breast cancer risk. In addition to watercress, other good sources of folate include spinach, asparagus, and papaya.
After Tom Ram, a Tier 3+ trainer at Equinox Wall St., took my body measurements, performed some tests of flexibility, and then had me step on the InBody 770, an impedance-based scale, my state of health (and fitness) became clearer: I had excess body fat, which I already knew, but it was visceral body fat--inside the abdominal cavity—the bad kind that wraps around your organs and increases your risk of heart attacks and strokes.

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The Lenox Health Resort Weight Loss Program at Canyon Ranch eschews what it calls “tedious, cookie-cutter weight-loss directives and dreary menus” and instead offers guests a whole-person program with access to top experts in nutrition, exercise physiology, life management, and lifestyle medicine – all working to tailor the best weight loss plan for each guest. Stays range from 1 week and longer, with an all-inclusive no-tipping plan with meals and lodging.
Slimming Studios offers an array of enhanced weight loss products and services at centers across North Texas, and through trusted medical partners. Slimming Studios provides medical & noninvasive weight loss procedures, dietary supplements, weight control snacks, diet & workout plans, and weight loss counseling. Its purpose is to offer clients the very best in enhanced weight loss so they don’t have to wait to lose weight.

“The weight starting coming off,” she says. “I remember I lost 9 lbs. my first week. After I had lost 100 lbs., I started trying more adventurous exercise. I became fascinated by fitness and seeing what new things I could accomplish with my body. I do things that I never dreamed were possible like running races, lifting heavy weights, and completing a sprint triathlon.”
Sure, trampolines are built for kids, but as an adult, using one for rebounding is a great way to flatten your tummy and get rid of excess fat. “Not only is it a great cardio workout (which is the first step to tightening up your midsection) but it makes your core work like crazy so you are getting the cardio plus the toning: everything you need for a tight tummy!” explains Hope Pedraza, a Certified Personal Trainer through American College of Sports Medicine in 50 Ways to Shrink Your Belly. To get a comprehensive workout using a mini trampoline, Pedraza suggests jumping, lifting your knees up high, twisting, adding some light weights to move around while you’re jumping, and moving in all directions in different planes.

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There’s a reason Eat This, Not That! hired celebrity trainer Mark Langowski to develop Eat This, Not That! for Abs, our e-book system for getting a six-pack in six weeks: He said it wouldn’t include a single sit-up. “I have been a personal trainer for over 13 years—during this time, I have learned a lot about a lot, but the most important topic that I discovered was 10 years ago when I found out how damaging sit-ups are to the discs in my spine,” he told us. “It was after listening to genius professor Stuart McGill, who is head of spine biomechanics at the University of Waterloo, that I realized I had been doing more harm to myself and my clients by having them do traditional sit-ups.” Instead, “throughout the workout section of the Eat This, Not That! For Abs, I explain how to train the entire body in a way that is activating the core muscles in every exercise you do. A squat may look like a leg exercise


Have you ever been shamed for your body? By others or even by yourself? I know the answer. I took photos with my dear friend @mckeelycreative before I came here on this journey and as I was scrolling through them I stopped at this one. My stomach. Hanging out. Right there. I knew right away it would be one of the pictures that never made it to the light of day but then I thought - you know what? That belly is a part of me no matter what. No matter how much weight I lose this is a moment in my life when I was happy and starting to make changes for myself. The beginning. It started in this body and this joyful belly laughing moment was a part of it. I’m celebrating it. I won’t be a better person with a flatter stomach. And that’s not the reason I’m here. I’ve always covered up when my shirt would slide up or my sides would stick out. I felt bashful that my soft rolling skin was coming out to see the light of day. In front of others. I once was getting a manicure and when I sat down in the tiny chair the back of my shirt lifted up and my sides stuck out. My nails were wet and I didn’t even try to adjust but the nail technician came over and did it for me and smiled and giggled and pulled my shirt down over my sides for me. She was embarrassed for me. I could tell. And I was embarrassed because she was. Never again. No shame. Let it out. All of it. Before you look at your next picture that you’re not happy with and shame yourself for your body and say how “fat” you look and feel, remember this belly, my belly. No matter how my body transforms in the future and how it’s changed over the last three months I want to be proud of me at all stages shapes and sizes. I will never shame her, the woman in this photo and her body. No matter how I grow and change. I won’t forget her. She was happy just as she was there and ready for a change. That’s why I’m posting this beautiful accident, a real moment among the other ones that are more polished and don’t show as much skin. A beautiful picture doesn’t have to be perfect. Celebrate all versions of ourselves and continue to be better because we want to be an deserve to be not just because of how we look❤️
Have you ever been shamed for your body? By others or even by yourself? I know the answer. I took photos with my dear friend @mckeelycreative before I came here on this journey and as I was scrolling through them I stopped at this one. My stomach. Hanging out. Right there. I knew right away it would be one of the pictures that never made it to the light of day but then I thought - you know what? That belly is a part of me no matter what. No matter how much weight I lose this is a moment in my life when I was happy and starting to make changes for myself. The beginning. It started in this body and this joyful belly laughing moment was a part of it. I’m celebrating it. I won’t be a better person with a flatter stomach. And that’s not the reason I’m here. I’ve always covered up when my shirt would slide up or my sides would stick out. I felt bashful that my soft rolling skin was coming out to see the light of day. In front of others. I once was getting a manicure and when I sat down in the tiny chair the back of my shirt lifted up and my sides stuck out. My nails were wet and I didn’t even try to adjust but the nail technician came over and did it for me and smiled and giggled and pulled my shirt down over my sides for me. She was embarrassed for me. I could tell. And I was embarrassed because she was. Never again. No shame. Let it out. All of it. Before you look at your next picture that you’re not happy with and shame yourself for your body and say how “fat” you look and feel, remember this belly, my belly. No matter how my body transforms in the future and how it’s changed over the last three months I want to be proud of me at all stages shapes and sizes. I will never shame her, the woman in this photo and her body. No matter how I grow and change. I won’t forget her. She was happy just as she was there and ready for a change. That’s why I’m posting this beautiful accident, a real moment among the other ones that are more polished and don’t show as much skin. A beautiful picture doesn’t have to be perfect. Celebrate all versions of ourselves and continue to be better because we want to be an deserve to be not just because of how we look❤️
In one four-week Spanish study, researchers found that eating a calorie-restricted diet that also included four weekly servings of legumes aided weight loss more effectively than an equivalent diet sans the pulses. Those who consumed the legume-rich diet also saw improvements in their bad LDL cholesterol levels and systolic blood pressure. Next time you’re cooking something starchy for dinner, consider eating fiber and protein-packed lentils instead.
Though many believe chewing gum keeps you from mindlessly eating, the minty treat has its own drawbacks that can lead to a bigger belly. Not only does chewing gum cause you to swallow tummy-bloating air, many gums also contain sugar alcohols and artificial sweeteners like sorbitol and xylitol that can cause bloat. If you have to have something to chomp on, go for an organic variety like Glee gum or Simply gum instead. They’re still low-cal, but they don’t use those sweeteners that’ll make you puff up.

More than half of the attendees at In:spa are repeat visitors thanks to a realistic, holistic approach to wellness. Cal Reiet, a fantastically smart 15-bedroom manor house, provides the perfect base. Programmes are a mix of combined activities (HIIT workouts, a 10km coastal hike, yoga) and more tailored, emotionally nourishing sessions (nutrition advice and massages). It's not particularly flexible, but relinquishing decision-making for a week is hugely relaxing, as is exercising in such lovely surroundings. At the heart of the experience is the food, a menu of mainly vegetarian dishes such as seed bread slathered in almond butter and avocado, turmeric and pea salad. It's a programme that will keep making you feel better, even long after you have left.
Carb crazy? Consider this: Refined carbohydrates, such as bread, potatoes and rice, create a surge in insulin that in turn drives down your resting metabolic rate, explains Aronne. "It's important to keep carbohydrates in your diet, but really focus on fruits, vegetables and whole grains, which have less of an effect on insulin levels," he explains. And when buying whole-grain breads and cereals, make sure the first ingredient listed is whole wheat, whole oat or cracked wheat.
Whether or not you’re specifically aiming to cut carbs, most of us consume unhealthy amounts of sugar and refined carbohydrates such as white bread, pizza dough, pasta, pastries, white flour, white rice, and sweetened breakfast cereals. Replacing refined carbs with their whole-grain counterparts and eliminating candy and desserts is only part of the solution, though. Sugar is hidden in foods as diverse as canned soups and vegetables, pasta sauce, margarine, and many reduced fat foods. Since your body gets all it needs from sugar naturally occurring in food, all this added sugar amounts to nothing but a lot of empty calories and unhealthy spikes in your blood glucose.
The tools and information on the Weight Loss Resources site are intended as an aid to weight loss and weight maintenance, and do not offer medical advice. If you suffer from, or think you may suffer from, a medical condition you should consult your doctor before starting a weight loss and/or exercise regime. If you decide to start exercising after a period of relative inactivity you should start very slowly and consult your doctor if you experience any discomfort, distress or any other symptoms. If you feel any discomfort or pain when you exercise, do not continue. The tools and information on the Weight Loss Resources site are not intended for women who are pregnant or breast-feeding, or for any person under the age of 18. Copyright © 2000-2018 Weight Loss Resources Ltd. All product names, trademarks, registered trademarks, service marks or registered service marks, mentioned throughout any part of the Weight Loss Resources web site belong to their respective owners.
Weight training is the ultimate way to burn calories fast. "A pound of muscle burns up to nine times the calories of a pound of fat," explains Richard Cotton, M.A., chief exercise physiologist for myexerciseplan.com. Weight training increases your resting metabolic rate, which is the number of calories you burn while sitting on your butt. What's more, it gives your metabolism an added boost after you exercise, staying in overdrive for up to two hours after the last bench press, according to a study published in Medicine & Science in Sports & Exercise. Strapped for time? Try these quick moves: squats, bench step-ups, lunges, push-ups, pull-ups and planks. In a pinch, just do single sets of 10 for each exercise — you'll get optimal results for the time invested.
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Grazing is a surprisingly good idea because it helps you avoid metabolic slowdown. "Your body will be tricked into thinking it's constantly eating, so it will never slow your metabolism down," explains Bauer. Aim for five small meals (200 to 500 calories) a day rather than three large ones. Also try not to go more than four hours without eating — if you eat breakfast at 7am, for example, have a snack at 10am, lunch at noon, another snack at 3pm and dinner at 7pm.


The American Heart Association recommends that the amount of added sugar consumed in a day shouldn’t exceed 25 grams for women and 37.5 grams for men, but since the sweet stuff is in everything from bread to tomato sauce, most Americans aren’t adhering to those guidelines and they’re fatter for it. In a review of 68 clinical trials and studies, New Zealand researchers reported in the British Medical Journal that increasing sugar intake meant increasing body weight while reducing sugar meant reducing body weight. Additional research has shown that cutting back on the granular stuff is one of the fastest ways to lose weight.
My name is Rita Jean. I am so thankful for Fit Body Weight Loss. It has given me new hope that I will lose the weight once and for all. Its been 40 years of weight struggles for me and finally I found something that actually works for me. lost over 20 pounds this month, and best of all, for the first time in many years my blood sugar is at a healthy level. My doctors are pleased with my results and I am thrilled being able to do the things I love again, like sing for my church and even exercise daily! That alone is a miracle! With the help of my Coach, Christina Jordan, I have left those dark days behind me where I was too tired and lacked any motivation or self control to take care of myself. I am in control and working towards my goals now! I thank God for Fit Body and for my newly returned health!
How would you like to take all the great weight-loss results you’ve just read about—and double them? That’s what happens when you supplement your diet with a combination of vitamin D and calcium, according to a recent Nutrition Journal study. Just four weeks into the 12-week experiment, subjects who had taken these two nutrients—found in abundance in some Greek yogurt—lost two times more fat than the other group!
The American Heart Association recommends that the amount of added sugar consumed in a day shouldn’t exceed 25 grams for women and 37.5 grams for men, but since the sweet stuff is in everything from bread to tomato sauce, most Americans aren’t adhering to those guidelines and they’re fatter for it. In a review of 68 clinical trials and studies, New Zealand researchers reported in the British Medical Journal that increasing sugar intake meant increasing body weight while reducing sugar meant reducing body weight. Additional research has shown that cutting back on the granular stuff is one of the fastest ways to lose weight.

Sleep apnea, in which you actually stop breathing during sleep, is linked with being overweight or obese. “Sleep apnea is caused, in part, when extra tissue accumulates around the upper airway, which can change its shape and make the throat more likely to collapse during sleep,” says sleep expert Richard Shane, PhD, creator of the Sleep Easily method. In one study, 88 percent of people who lost 33 or more pounds were effectively cured. According to Harvard Medical School, losing just 10 percent of your body weight can drastically reduce sleep apnea symptoms—and in some cases, weight loss can even cure the condition. Don’t miss these other signs your weight gain means your health is in trouble.


As you mention, HS wrestlers (also, many other grappling sport athletes), boxers, etc. aren’t given the full 24 hours to recover from depletion. Many of these sports have mat-side weigh-ins. Others are just given a few hours after weigh-in to replenish. For these sports, athletes are best served focusing on year-round nutrition strategies, ones that help them stay at a weight very close to the weight they’d like to compete at.
Weight loss can be intentional, such as from dieting and exercise, or unintentional and be a manifestation of illness. Weight loss can result from a decrease in body fluid, muscle mass, or fat. A decrease in body fluid can come from medications, fluid loss, lack of fluid intake, or illnesses such as diabetes. A decrease in body fat can be intentionally caused by exercise and dieting, such as for overweight or obesity. Weight loss is normal after pregnancy. Other causes of weight loss include, but are not limited to, cancer, viral infection (such as CMV or HIV), gastroenteritis, parasite infection, depression, bowel diseases, and overactive thyroid (hyperthyroidism).
The truth is there is no “one size fits all” solution to permanent healthy weight loss. What works for one person may not work for you, since our bodies respond differently to different foods, depending on genetics and other health factors. To find the method of weight loss that’s right for you will likely take time and require patience, commitment, and some experimentation with different foods and diets.
Aim to get at least seven hours of sleep each night. Try going to bed and waking up at the same time each day. Get these seven tips for the best sleep ever! Why? Ever notice how you start to crave donuts and drive-thru breakfasts when you’re exhausted? When you don’t get enough sleep, your hormones are thrown out of balance. Running on no sleep can actually drive up the hormones that make you want to eat, while pushing down the hormones that signal for fullness—and that’s a recipe for weight gain. When you’re well-rested, it’s much easier to make healthy decisions and stay on track.
Not like you needed another reason to fall in love, snuggle up with your sweetie, kiss or get it on. Harvard Medical School researchers found that all of those things can aid weight loss. How? Lovey-dovey feelings cause levels of the hormone oxytocin to increase, which in turn, decreases appetite. For even more weight loss hacks, check out these 20 Weight Loss Tricks You Haven’t Tried.
Want to lose eight pounds in a week?  Who wouldn't?  The problem is that fast weight loss is not the same as healthy weight loss. Rapid weight loss often includes muscle tissue, which is denser than fat and which helps burn calories. Losing muscle also slows down the body's metabolic rate so that it is more efficient at holding onto the calories you do take in.That causes people to gain all the weight they lost, and then some. American spas learned this the hard way. In the sixties, they started out as "fat farms," where women went to lose weight fast by eating 800 calories a day, exercising for hours, and obsessively measuring.
The scientists ferried 20 overweight, middle-aged men by train and cable car to a research station perched 1,000 feet below the peak of Germany's highest mountain, Zugspitze. During the week-long stay, the men could eat and drink as much as they liked and were forbidden from any exercise other than leisurely strolls. The team measured the men's weight, metabolic rate, levels of hunger and satiety hormones before, during, and after their mountain retreat After a week up high, the subjects lost an average of 3 pounds. A month later, they were still 2 pounds lighter. The scientists' data showed this was likely because they ate about 730 calories less at high altitudes than they did at normal elevations. They may have felt less hungry, in part, because levels of leptin, the satiety hormone, surged during the stay, while grehlin, the hunger hormone, remained unchanged. Their metabolic rate also spiked, meaning they burned more calories than they usually did. A high-altitude weight loss strategy could be viable, though studies have shown peoples' appetites bounce back after about six months at high elevation, Leissner said. “If you could do intermittent periods for one week, then go down, and then go back up, this might actually be helpful.”
Evening eating is often the most problematic for women, as it can be related more to emotional issues than to real hunger. You're tired, lonely, bored, anxious; you want to relax or reward yourself after a tough day. But indulge too many nights in a row and that temporary water gain becomes permanent fat gain — and fatter abs, rather than the flat abs you want.
We all know that nuts are a great healthy snack but often people get scared by the fact that too many can be bad news - when to stop?!? Well the American Pistachio Growers say that 37 pistachios are the equivalent of LESS THAN 100 calories! Plus nuts like almonds are high in fibre, which helps you feel satisfied in between meals. We don't need another excuse...

Looking for the easiest possible way to lose weight? Grab your pajamas early and log some extra Zzzs! According to researchers, getting eight and a half hours of shut-eye each night can drop cravings for junk food a whopping 62 percent and decrease overall appetite by 14 percent! Mayo Clinic researchers note similar findings: In their study, adults who slept an hour and 20 minutes less than the control group consumed an average of 549 additional calories daily. That’s more calories than you’ll find in a Big Mac!
Did you know that running for a straight hour on the treadmill is about three times LESS effective than just 15 minutes of interval training? If that isn't enough, the American College of Sports Medicine Annual Meeting says that doing just two weeks of high-intensity interval training will improve your aerobic fitness as much as about seven weeks of endurance training.
Not in an extreme, Atkins sort of way, but having a little protein at every meal fires up your metabolism. "Your digestive system uses more energy to break it down, so you burn more calories," explains Lisa Dorfman, R.D. However, keep protein levels to between 20 and 35 percent of your diet; eating too much of it can cause kidney strain and may cause your body to store too much fat.
Plasma concentrations of PFOS, PFOA, perfluorononanoic acid (PFNA), perfluorohexanesulfonic acid (PFHxS), and perfluorodecanoic acid (PFDA) were measured at baseline only, using a sensitive and reliable method based on online solid phase extraction and liquid chromatography coupled to a triple quadropole mass spectrometer [41], with minor modifications. Due to the long elimination half-lives of the PFASs and incomplete samplings, we did not measure plasma PFAS levels during the trial. For all major PFASs, the concentrations were above the limit of detection (0.05 ng/ml), and the inter- and intra-assay coefficients of variation were <6.3% and <6.1%, respectively.

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