I have always had an athlete’s physique until my sophomore year of college when I gained about 50 lbs. Since then I’ve tried everything to lose the weight only to fail… Until I started the Fit Body Weight Loss Program and the weight came off easily! I dropped 9 lbs in just the first week! I went from 203 lbs to 182 lbs and dropped from a tight size 16 to a size 12 in just a little over a month! I’m now wearing clothes that I haven’t been able to fit into for over 2 years and now they are even loose on me! This program really worked for me when nothing else did! 

Guests who sign up at this lavish resort can expect an intense, yet detoxifying experience. The weeklong boot camp ($5,800 during the summer) incorporates nine to ten hours a day of stimulating mind and body exercises. Each morning begins with a four-to-five hour group hike through a mountain trail followed by a fresh, organic meal. The resort implements a 1,500-calorie a day diet that excludes alcohol, caffeine and processed sugars. After lunch, guests will participate in low-impact, moderate activities, such as water aerobics, weight training and yoga. The ranch’s program includes accommodation as well as activities and meals. The resort accommodates 16 guests at a time and provides a private room with a queen size bed and bathroom for each guest. As a part of the resort’s detoxifying approach, it does not provide Wi-Fi or cell service, however guests can access a communication center for a limited time each day.
Have you ever found yourself trying not to eat that piece of chocolate cake you’ve been craving all day, so you end up eating everything else in its path (and probably still eat the cake, too)? One way to enjoy some of the foods you want without reaping the negative consequences is to allow yourself a few bites. This tactic has helped many of my patients who are going on vacation or dealing with the struggle of powerful cravings.
Not in an extreme, Atkins sort of way, but having a little protein at every meal fires up your metabolism. "Your digestive system uses more energy to break it down, so you burn more calories," explains Lisa Dorfman, R.D. However, keep protein levels to between 20 and 35 percent of your diet; eating too much of it can cause kidney strain and may cause your body to store too much fat.
But perhaps the biggest change has been in my mindset. I’m learning that health is all about balance, which I’ve always known but to be here and live it has been eye opening and comforting seeing it firsthand. It’s so easy to fall into an "all or nothing" mindset, thinking that if I couldn't do everything perfectly then it wasn't worth trying or if I “messed up” then it wasn’t worth continuing on.
I started my journey this summer with 19% body fat, at the upper range of what would be considered acceptable for percentage body fat. The measurement is based on the principle of impedance, the transmission of electrical current through various body tissues, with fat creating the most resistance and muscle the least, based on its higher water content.

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HIIT (High Intensity Interval Training) is a type of workout becoming more and more widespread through gym classes and exercise videos such as P90X and Insanity. This way of exercising yields great results because they combine high intensity movements with very little rest in between. As a result, you can fit in a fantastic fat-burning workout in a short amount of time.
Fit Body Weight Loss is an all-natural, holistic weight loss clinic based in Gilbert, Arizona. We work with clients from all over America. We offer a therapeutic nutrition program that combines REAL, nutrient dense foods, supplementation, coaching, education and individualized support to safely and reliably achieve healthy, RAPID results. Place your order online and it will be conveniently delivered to your home or office within days.
In a study conducted by Callaway, people who skipped breakfast or lunch and ate their largest meal later in the day had lower metabolisms. So by eating light at night you'll receive a double benefit: You'll wake up with a flatter tummy, and you'll also have a better appetite for a fiber-rich breakfast, which sets you up for a day of healthful eating. Some diet tips to get you started:
That’s because the tough love formula on Mountain Trek’s one- and two-week programs ($4,500 and $8,850 Canadian) pays off: Metabolisms rise and pants get loose. And then it all starts to just feel good. By the fourth day or so, the 6:30am yoga classes, sensibly portioned meals, challenging four-hour hikes—in which the encouraging and attentive guides insist you speed up if you’re able to speak in full sentences—and even health lectures start adding up to something energizing. Cortisol is losing! Metabolism-boosting hormones like HGH and DHEA are winning!
Not only is drinking lemon water a healthy, low-calorie alternative to soda or juice, but lemons themselves have also been shown to contribute to weight loss. Just one of the citrus fruits contains an entire day’s worth of vitamin C, a nutrient that has the power to reduce levels of cortisol, a stress hormone that triggers hunger and fat storage. Additionally, lemons also contain polyphenols, which researchers say may ward off fat accumulation and weight gain. Believe it or not, even the peel is beneficial because it is a potent source of pectin—a soluble fiber that’s been proven to help people feel fuller for longer. According to a study published in the Journal of the American College of Nutrition, participants who ate just 5 grams of pectin experienced more satiety.

Limited to just 15 people each week, the Sunday to Saturday stay at the Ranch at Live Oak Malibu is designed to deliver sustainable results through a strict, “no-options” philosophy.   Tough workouts, physical endurance, yoga, and hiking are key elements of the program as well as the vegetarian cuisine sourced on the property. No cell phones are permitted and while accommodations are beautifully appointed, you won’t find televisions to distract you from activity.
A different way of viewing weight loss identifies the problem as not one of consuming too many calories, but rather the way the body accumulates fat after consuming carbohydrates—in particular the role of the hormone insulin. When you eat a meal, carbohydrates from the food enter your bloodstream as glucose. In order to keep your blood sugar levels in check, your body always burns off this glucose before it burns off fat from a meal.
Is your obsession with Reese’s and Pringles derailing your weight loss efforts? It might be if you’re not using the self-checkout kiosks at the grocery store. Let us explain: According to a study by IHL Consulting Group, impulse purchases dipped 32.1 percent for women and 16.7 percent for men when they scanned their items and swiped their credit card on their own. Although not all impulse buys are bad for your belly, a whopping 80 percent of candy and 61 percent of salty-snack purchases are unplanned.

Using a layered approach is another great way to build a good veggie habit. For example, start with a food you already enjoy — say, pasta — and layer some veggies into your bowl. This can help you explore a new food with one you already love eating, and from there, you can try new ways to savor it. Take spinach, for instance. After trying it with pasta, you may want fold it into an omelet or another favorite food, or explore it on its own with different cooking techniques (sautéed or steamed) or different flavor additions (garlic or golden raisins). The possibilities are limitless!


Data are least-square means, adjusted for age, race, education, smoking, alcohol consumption, physical activity, menopausal status (women only), hormone replacement therapy (women only), dietary intervention group, baseline free T3 and free T4 levels, and baseline BMI. PFAS, perfluoroalkyl substance; PFDA, perfluorodecanoic acid; PFHxS, perfluorohexanesulfonic acid; PFNA, perfluorononanoic acid; PFOA, perfluorooctanoic acid; PFOS, perfluorooctanesulfonic acid; T3, triiodothyronine; T4, thyroxine.


Carb crazy? Consider this: Refined carbohydrates, such as bread, potatoes and rice, create a surge in insulin that in turn drives down your resting metabolic rate, explains Aronne. "It's important to keep carbohydrates in your diet, but really focus on fruits, vegetables and whole grains, which have less of an effect on insulin levels," he explains. And when buying whole-grain breads and cereals, make sure the first ingredient listed is whole wheat, whole oat or cracked wheat.
Sure, you certainly need to drink plenty of water to help expedite the process of ridding your body of excess sodium, you can (and should!) also consume high-water content foods. Reach for cucumbers, tomatoes, watermelon, asparagus, grapes, celery, artichokes, pineapple, and cranberries — all of which contain diuretic properties that will also help you stay full due to their higher fiber content.
My advice is to not using anything from this article for sustained weight loss. Instead, I’d love to see you google “Precision Nutrition Get Shredded Diet”, which provides a rapid fat loss plan that’s based on actual fat (not water) loss. Another approach would be to google “Precision Nutrition Intermittent Fasting”, which provides another alternative.
"Tight glycemic control is necessary to maintain health and to prevent disease," Ellen Blaak, a professor of fat metabolism and physiology at Maastricht University, wrote in a review of studies published in the journal Obesity Reviews. Her study found links between poorly controlled blood-sugar levels and obesity, Type 2 diabetes, and heart disease.
Hilton Head Health (H3) is a destination health resort offering customized programs focusing on fitness, nutrition and SELF (Stress management, Empowerment, Longevity, and Fulfillment).   Graduates of the programs continue their lifestyle change with the H3@Home program and receive continued support and structure at home.  The H3@Home blog is also available for readers to benefit from recipes, tips, and workout plans.
The Wisconsin native also struggled with health issues. In her early teens she was diagnosed with polycystic ovarian syndrome (PCOS) and sleep apnea. “It is extremely difficult to lose weight and maintain weight loss with PCOS,” says Stolfi, who hit her highest weight of 286 lbs. at age 18. “And the lack of good sleep caused me to be lethargic, which meant I didn’t have the energy to work out.”
Virtual gastric band uses hypnosis to make the brain think the stomach is smaller than it really is and hence lower the amount of food ingested. This brings as a consequence weight reduction. This method is complemented with psychological treatment for anxiety management and with hypnopedia. Research has been conducted into the use of hypnosis as a weight management alternative.[16][17][18][19] In 1996 a study found that cognitive-behavioral therapy (CBT) was more effective for weight reduction if reinforced with hypnosis.[17] Acceptance and Commitment Therapy ACT, a mindfulness approach to weight loss, has also in the last few years been demonstrating its usefulness.[20]
Join our comprehensive weeklong JumpStart weight loss retreat to lose weight and you will leave with a strategy for continued success. Or take advantage of our intensive month-long LoseWell program. These weight loss programs provide a holistic approach, helping you overcome the mental and physical challenges that may have prevented you from reaching your wellness goals.
One of the best new examples of a weight loss spa is Skyterra Wellness Retreat about an hour from Asheville, North Carolina. It's a small program that encourages longer stays (one to four weeks) where everyone eats the same delicious, healthy meals at communal tables. They exercise together, attend lectures together, even go into Asheville for a bit of chocolate and coffee at French Broad together!

Many women believe that drinking too much water will give them puffy abs, but just the opposite is true. "Even though we associate water with being bloated, drinking more water can help to flush sodium out of the body, and that reduces the bloat," said Jeff Hampl, Ph.D., R.D., nutrition researcher and assistant professor at Arizona State University.

The Dietary Guidelines Advisory Committee dropped their longstanding recommendation that we should limit dietary cholesterol. Decades of research have shown that it has little effect on blood cholesterol levels, and the government’s outdated recommendations have done little more than send scrambled messages about the pros and cons of eating eggs and shrimp. So go ahead and scramble up an omelet—with the yolk. Eating the entire egg is beneficial to your body because it contains metabolism-stoking nutrients, including fat-soluble vitamins, essential fatty acids, and choline—a powerful compound that attacks the gene mechanism that triggers your body to store fat around your liver. To learn more about the flat-belly benefits of eggs, check out these What Happens to Your Body When You Eat Eggs.
The CDC found that the average adult consumes about 100 calories worth of alcohol daily, but favoring a glass of wine instead of beer or sugary cocktails can drastically reduce that figure and make your waistline slimmer. Plus, wine is a healthy alternative for those who don’t want to give up booze entirely. In addition to having fewer calories than most alcoholic beverages, red wine in particular is a good source of those waist-shrinking flavonoids that are also found in red fruits. Resveratrol, a particular flavonoid found in red wine, is believed to have heart-healthy benefits because it helps prevent blood vessel damage and reduces your bad LDL cholesterol. Just remember to imbibe in moderation.
Jump up ^ Mann, T; Tomiyama, AJ; Westling, E; Lew, AM; Samuels, B; Chatman, J (April 2007). "Medicare's search for effective obesity treatments: diets are not the answer". The American Psychologist. 62 (3): 220–33. doi:10.1037/0003-066x.62.3.220. PMID 17469900. In sum, there is little support for the notion that diets ["severely restricting one’s calorie intake"] lead to lasting weight loss or health benefits.
Nutritionist, Emma Wight-Boycott says: “Skipping meals is disastrous for weight loss! It has a three-pronged impact - firstly it causes blood sugar imbalance which makes us crave sugar or carbohydrates within 2 hours of our regular meal time. Secondly it makes our bodies burn muscle, not fat, for energy. Finally the body has its own 'food clock' (like a body clock for time) that regulates our metabolism and this becomes sleepy or sluggish when it is out of routine (think shift workers or jet lag)."
If you have a big celebration or date coming up, you might think it makes sense to “save” your calories for when it’s time to let loose, but this technique is rarely effective and could actually be hindering your ability to lose weight. “Although this makes sense in theory—consuming fewer calories total per day—it rarely works out as cleanly as we like,” Lisa Hayim spelled out for us in The 30 Worst Diet Mistakes You’re Making. “By the time you get to the date, and have a drink or two, the feelings of extreme hunger rush in, and you’re grabbing for whatever you can get your hands on, which is usually foods high in calories and fat. You’re so hungry, you may even end up consuming more than a day’s worth of calories in one sitting! Plus, with alcohol in your system, your body is less able to efficiently metabolize the calories,” explains Hayim. “Instead, consume normal meals throughout the day, arrive at your date cool, calm, and collected, and enjoy your cocktail and eat responsibly.”
Cheese isn’t traditionally thought of as something you consume to encourage weight management, but calcium-rich Parmesan, when eaten in moderation, can help stave off sugar cravings that can easily lead to weight gain. How does that work, you ask? The native Italian cheese contains the amino acid tyrosine (remember that?) which has been shown to encourage the brain to release dopamine without any unhealthy insulin spikes. What’s more? The combination of calcium and protein present in dairy products such as Parmesan has been found to increase thermogenesis—the body’s core temperature—and thus boost your metabolism.
Finally decided to venture out for a run? Snack on some beets before you hit the pavement. A study published in the Journal of the Academy of Nutrition and Dietetics found that runners who ate baked beets before a 5K race ran 5 percent faster. Researchers suspect this is because beets are high in nitrates, a natural chemical that increases endurance and lowers blood pressure.
More than half of the attendees at In:spa are repeat visitors thanks to a realistic, holistic approach to wellness. Cal Reiet, a fantastically smart 15-bedroom manor house, provides the perfect base. Programmes are a mix of combined activities (HIIT workouts, a 10km coastal hike, yoga) and more tailored, emotionally nourishing sessions (nutrition advice and massages). It's not particularly flexible, but relinquishing decision-making for a week is hugely relaxing, as is exercising in such lovely surroundings. At the heart of the experience is the food, a menu of mainly vegetarian dishes such as seed bread slathered in almond butter and avocado, turmeric and pea salad. It's a programme that will keep making you feel better, even long after you have left.

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The primary strength of the current study is that the cause of weight changes was well characterized. Unlike previous observational studies in which reasons for weight changes were usually unknown, this weight-loss trial applied energy restriction to induce the weight changes. Moreover, repeated measurements of body weight, RMR, thyroid hormones, leptin, and other metabolic biomarkers allowed documentation of longitudinal associations between PFAS exposures and changes in these parameters during the weight-loss and weight regain periods.
There are two versions of this diet: one is where you supplement healthy, daily meals with strawberries, for their natural health benefits (they contain high levels of vitamin C, folic acid and potassium), but the more extreme version of the diet is the strawberry fast, in which the person lives on nothing but strawberries to lose weight quickly. While strawberries are very healthy, eating them alone means that you miss out on other vital ingredients you get from a balanced diet. Strawberries are also very high in sugar, just 100g of strawberries contains about 7g of sugar – that’s almost two teaspoons. And after Kim Kardashian was reported to be trying the strawberries-only diet, we’re sure there’ll be a few people ready to follow in her slimming footsteps…
Also some research shows that the human body is primed to consume most of its calories during daylight hours. But the lifestyle is problematic for many: Because family meals and dinners with friends often are scheduled for after sunset, “people who try to stop eating after 7pm can’t do it every day for the rest of their lives,” says Dr. Seltzer, who supports an alternative strategy: Eating a hearty meal at your regular dinnertime.
The Dietary Guidelines Advisory Committee dropped their longstanding recommendation that we should limit dietary cholesterol. Decades of research have shown that it has little effect on blood cholesterol levels, and the government’s outdated recommendations have done little more than send scrambled messages about the pros and cons of eating eggs and shrimp. So go ahead and scramble up an omelet—with the yolk. Eating the entire egg is beneficial to your body because it contains metabolism-stoking nutrients, including fat-soluble vitamins, essential fatty acids, and choline—a powerful compound that attacks the gene mechanism that triggers your body to store fat around your liver. To learn more about the flat-belly benefits of eggs, check out these What Happens to Your Body When You Eat Eggs.
Getting clear about why you want to lose weight will provide you with the fuel to keep going when your resolve starts to weaken (as—let’s be honest—it inevitably will). “I’d encourage those who are thinking of getting healthier to take some time to reflect and journal about what matters most,” Shirley Mast, R.N., B.S.N., and Take Shape For Life Health Coach, tells SELF.
Forget fad diets and gastric bands, the latest innovation in the war on obesity is fat busting paintball sessions. A UK paintball company has announced what is believed to be the world's first ever paintball fitness classes. During the sessions, people wanting to get fit will be invited to dodge paintballs while running an assault course, while the paintball centre's staff attempts to shoot them.
Women who ate low-fat dairy products, such as non-fat yogurt and low-fat cheese three to four times a day lost 70 percent more fat than low-dairy dieters, according to a study published in the journal Obesity Research. "Calcium serves as a switch that tells your body to burn excess fat faster," explains study author Michael Zemel, M.D., director of the Nutrition Institute at the University of Tennessee in Knoxville. Sorry, but you won't reap the same benefits from calcium-fortified O.J. Research shows that you get the best results from dairy products themselves, not fortified foods. Aim for 1,200 mg, which includes about three servings of dairy a day.
Loads of research demonstrates people who log everything they eat — especially those who log while they're eating — are more likely to lose weight and keep it off for the long-haul. Start tracking on an app like MyFitnessPal when the pounds start sneaking up on you. It'll help you stay accountable for what you've eaten. Plus, you can easily identify some other areas of your daily eats that could use a little improvement when it's written out in front of you. 
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Pax intrantibus; Salus exeuntibus. This latin phrase is engraved above the entrance to Grayshott Health Spa.  It translates to Peace on Arrival and Health on Departure. Taking a naturopathic approach, Grayshott uses therapeutic treatments, scientific dietary protocols and eating practices to tackle health from the inside out.  The 7 – 21 day programs are strict, precise, and controlled and start a new chapter in health.
In addition to improving your health, maintaining a weight loss is likely to improve your life in other ways. For example, a study of participants in the National Weight Control Registry* found that those who had maintained a significant weight loss reported improvements in not only their physical health, but also their energy levels, physical mobility, general mood, and self-confidence. 

wow! 20-30 pounds weight loss in just 5 days?? just as I saw the title I was a bit curios about it as to how can you do that for just 5 days. It is really hard work for the boys who really wanted to be fit like that in those pictures posted. i believe if you are really into something like doing these why not? If you are just determined and persistent in everything I am sure that you can really loss weight for 20-30 pounds or even more. i believe you just have to believe in yourself. GO FOR GOLD

Afternoons often include a TRX strength class or cardio boxing, both of which I have fallen in love with, or meeting up with my trainer for a personal training session. One of my primary goals here is to be strong so we're really working on strength training. I wrap up with hip-hop dance or yoga. Even though they’re totally different classes, each bring me joy. If that sounds like a lot of exercise, it is, but even though it's more movement than I've ever done in my life, it's always fun and there's no pressure to do it. I don't have to do this many classes and some days I choose to rest.


Slimming Studios offers an array of enhanced weight loss products and services at centers across North Texas, and through trusted medical partners. Slimming Studios provides medical & noninvasive weight loss procedures, dietary supplements, weight control snacks, diet & workout plans, and weight loss counseling. Its purpose is to offer clients the very best in enhanced weight loss so they don’t have to wait to lose weight.
The Danubius Health Spa Resort and Grand Spa Hotel in Piešťany is reknowned for its healing treatments offered to Europeans and visitors for more than 100 years.  Its natural springs’ healing properties are represented by a “crutch breaker”, the iconic symbol of Spa Piešťany. Danubius weight loss programs and packages include accommodations, meals, physician consults and exams, fitness evaluation, laboratory tests, medical reports upon departure, and more.
Table 1 shows the baseline characteristics of the study participants. PFOS and PFOA were the dominant PFASs. The median (interquartile range) plasma concentration was 24.5 (16.2–37.0) ng/ml for PFOS, 4.5 (3.3–6.3) ng/ml for PFOA, 2.4 (1.5–3.6) ng/ml for PFHxS, 1.5 (1.0–2.4) ng/ml for PFNA, and 0.37 (0.27–0.52) ng/ml for PFDA. At baseline, significant inter-correlations were observed between PFOS, PFOA, PFHxS, PFNA, and PFDA (rs ranged from 0.38 to 0.85) (S2 Table), although no particular pattern of PFAS mixture was identified in the factor analysis. After multivariate adjustment, PFOS, PFOA, and PFNA concentration were all positively associated with insulin, HOMA-IR, diastolic blood pressure, and free T3 (rs ranged from 0.10 to 0.18, all P < 0.05) at baseline. In addition, certain PFASs (e.g., PFHxS and PFDA) were positively associated with some of the variables, including visceral fat mass, systolic blood pressure, glucose, triglycerides, LDL cholesterol, free T4, total T4, and leptin (rs ranged from 0.08 to 0.24, all P < 0.05) (S2 Table). No PFASs were correlated with body weight, waist circumference, BMI, or RMR at baseline.
Plain and simple: We just don't feel full by liquid calories in quite the same way as we do real food. Drinking a juice or caramel coffee drink, for instance, won't make you feel full the way eating a bowl of veggie- and protein-packed stir-fry will. So monitor your intake of juice, soda, sweetened coffee and tea, and alcoholic beverages. If you consume each of those beverages during the day, you'll have taken in at least 800 extra calories by nighttime — and you'll still be hungry. (Incidentally, alcohol may suppress the metabolism of fat, making it tougher for you to burn those calories.) Some other ways to skip sugar? Check 'em out here.
A new German study found that when you drink 17 ounces of water (about two glasses) within a certain time frame, your metabolic rate shoots up by about 30 percent. Using these results, they estimate that by increasing your current water intake by 1.5 liters a day, a person would burn an extra 17,400 calories a year, resulting in about a five-pound weight loss. 

Ken has been in the healthcare industry for over 10 yrs where he started first as a Registered nurse and later as a Family Nurse Practitioner. He obtained his BSN from The University of Texas Medical Branch in 2008 and later a Masters of science in Nursing at Walden University. He loves and is dedicated to his patients. While not working, Ken enjoys traveling and some gardening. Ken is excited to help patients who are trying to live healthier lives by losing weight and having a wellness regimen.
In addition to coordinating with your dishes, the hues you surround yourself with while you chow down can impact your appetite. According to several studies, blue is an appetite suppressant. Scientists suspect this is because there aren’t many naturally-occurring blue-hued foods aside from blueberries and a handful of others. This behavior might also stem from our ancestors, who when foraging for food, stayed away from sources that were blue, black, and purple because they were believed to be poisonous. So buy some blue dishes, or freshen up your eating area with a blue tablecloth or placemats.
When you eat a high-sodium meal, say, from your favorite Chinese takeout at 3,000 milligrams per entree, your body responds by retaining water. This results in edema and, possibly, a rise in blood pressure. Sodium's visible traces are outlined the next day when you stand sideways in your full-length mirror: major B-L-O-A-T! Even though it's temporary, that's no consolation when you want to wear something revealing that day. Here are diet tips to lower your sodium intake:
The Danubius Health Spa Resort and Grand Spa Hotel in Piešťany is reknowned for its healing treatments offered to Europeans and visitors for more than 100 years.  Its natural springs’ healing properties are represented by a “crutch breaker”, the iconic symbol of Spa Piešťany. Danubius weight loss programs and packages include accommodations, meals, physician consults and exams, fitness evaluation, laboratory tests, medical reports upon departure, and more.
Avoiding salt doesn’t mean your food has to be bland. Experiment with using different herbs and spices. Try adding fresh cilantro and cumin to grilled fish, lemon and rosemary to chicken, or ginger and Chinese five spice to tempeh or beef. Pick up some spice blends from your local market to help add more spice to your life… just read the ingredients and make sure there’s no salt added.
Going way beyond your BMI–-a measure of your height and body weight--the scale calculates your percentage body fat, including your visceral fat area (VFA), which provides the most accurate measure of your risk for adverse cardiac events such as heart attack and stroke. BMI is not a true reflection risk of your cardiac risk since increased skeletal muscle mass in relation to height can elevate your BMI, falsely elevating cardiometabolic risk.
Plasma concentrations of PFOS, PFOA, perfluorononanoic acid (PFNA), perfluorohexanesulfonic acid (PFHxS), and perfluorodecanoic acid (PFDA) were measured at baseline only, using a sensitive and reliable method based on online solid phase extraction and liquid chromatography coupled to a triple quadropole mass spectrometer [41], with minor modifications. Due to the long elimination half-lives of the PFASs and incomplete samplings, we did not measure plasma PFAS levels during the trial. For all major PFASs, the concentrations were above the limit of detection (0.05 ng/ml), and the inter- and intra-assay coefficients of variation were <6.3% and <6.1%, respectively.

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