Every person has a different palate, a unique attitude toward food, and various likes and dislikes. That means you need to find a nutrition plan that works best for you. The phrase "healthy eating" gets thrown around a lot, but for many people, the changes needed to get there aren't as big as they think. It might just be replacing your usual snack for a healthier one, and fixing the one meal each day where you are most likely to overeat.
Why a top-pick? Many weight loss retreats develop their programs around a specific exercise schedule and predetermined nutritional goals. At Kripalu, participants spend more time delving into why they eat and what they can do to make healthier choices. Dieters don't go home with a specific meal plan to follow, but they have the opportunity to connect with others so that they can continue to build on the foundation that they establish at Kripalu.
My name is Rita Jean. I am so thankful for Fit Body Weight Loss. It has given me new hope that I will lose the weight once and for all. Its been 40 years of weight struggles for me and finally I found something that actually works for me. lost over 20 pounds this month, and best of all, for the first time in many years my blood sugar is at a healthy level. My doctors are pleased with my results and I am thrilled being able to do the things I love again, like sing for my church and even exercise daily! That alone is a miracle! With the help of my Coach, Christina Jordan, I have left those dark days behind me where I was too tired and lacked any motivation or self control to take care of myself. I am in control and working towards my goals now! I thank God for Fit Body and for my newly returned health!
Not like you needed another reason to fall in love, snuggle up with your sweetie, kiss or get it on. Harvard Medical School researchers found that all of those things can aid weight loss. How? Lovey-dovey feelings cause levels of the hormone oxytocin to increase, which in turn, decreases appetite. For even more weight loss hacks, check out these 20 Weight Loss Tricks You Haven’t Tried.
If you're calorie counting the chances are that you're going to be underestimating the damage - by a long way. If you didn't see Channel 4's Secret Eaters, you might not be aware of just how many secret calories we eat everyday that go unnoticed. Whether measuring the packet's calorie counting by the wrong servings or just missing the essential maths, a good rule of thumb is to add about 10 percent of what you think you're eating to get your actual calorie count.
Wondering why it's so hard to lose that muffin top? There are so many environmental and personal factors that contribute to our putting on pounds and it's not enough to enroll in the latest fad diet or jog for half an hour every day to ensure weight loss. To successfully lose weight and keep it off you have to look at everything from your nutrition and fitness to your sleep habits and stress management. This may seem like a lot but Mountain Trek program can work for you.
In a new study, Stanford University researchers put more than 600 overweight adults on either a healthy low-fat or low-carb diet. It turns out, participants had similar levels of weight loss success on each plan. Researchers looked for clues (such as insulin levels and gene patterns) to see if there are any factors that might make someone more successful on either diet, but after combing through the data, they were not able to make any connections. Since it may take years before scientists discover individual traits that could lead to more success on one plan compared to another, for now, we can learn a lot — and lose a lot! — by recognizing the dieting advice that all experts agree on.
Guests can have a sulfur mud bath under the stained-glass windows to target specific problem areas. The slimming program focuses not only on diet and fitness, but also on preventing damage to joints and to the body. Natural springs have been proven to aid in diseases such as rheumatism, and also give incredible energy and strength to anyone coming to kickoff a fitness lifestyle.
This is not a rough-around-the-edges weight loss boot camp that you will find at a discounted price on Groupon (though those have their value, too). Instead, this is a wellness retreat that teaches you how to move your body in a way that opens up attunement and respect for what it’s capable of. The movement will show you your own strength, both for the ability to move it and for the ability to commit to it. It’s an empowering experience to learn how to move your body. I promise that you will fall in love with your body when you realize just what it is capable of.
One Body helps you understand the critical question of how and why you may have gained weight, and then clearly lays out a plan to help you lose weight long term. You will be given a low carb, high protein diet and regimen, that will help shed pounds week after week. Many One Body patients lose up to 15-20 pounds the first month* and up to 2-5 pounds per week thereafter.
In the UK, up to 5% of the general population is underweight, but more than 10% of those with lung or gastrointestinal diseases and who have recently had surgery. According to data in the UK using the Malnutrition Universal Screening Tool ('MUST'), which incorporates unintentional weight loss, more than 10% of the population over the age of 65 is at risk of malnutrition. A high proportion (10-60%) of hospital patients are also at risk, along with a similar proportion in care homes.
losing weight after 45 years old
Lie down with forearms on the floor and elbows beneath shoulders. Feet should be flexed with toes on the floor. Rise up on your toes so that only your forearms and toes touch the floor. Your body should be a few inches off the floor in a straight line. Bring belly button to spine by contracting your deep abdominal muscles and tightening your buttocks and upper body.
New research from the University of Michigan Life Sciences Institute has determined that the popular holiday spice can help fight obesity thanks to cinnamaldehyde, an essential oil that gives cinnamon its flavor. According to researchers, cinnamaldehyde improves metabolic health by acting directly on fat cells, inducing them to start burning energy via thermogenesis. To work cinnamon into your diet, try sprinkling some on oatmeal or sipping on cinnamon tea.
Meatless Monday is more than just an alliteration; it’s an easy way to drop a few pounds. Numerous studies have shown that those who eat the least amount of meat are less likely to be obese, have lower BMIs, and lower body fat levels. Though it’s perfectly fine to eat meat a few times a week, these high-protein foods tend to fill you up before you can work your way over to the veggies, which possess fat-fighting, waist-trimming powers. Try spotlighting just greens and healthy grains in your meals a few times a month.
Several limitations should be considered as well. First, although we included men and women with a wide range of ages (30–70 years), participants in the current study were otherwise relatively homogeneous in terms of health status and body fatness because they were selected following narrow inclusion criteria. Therefore, it is unclear whether our findings can be extrapolated to more general populations. Second, we measured only the baseline plasma PFAS concentrations. However, given the long elimination half-lives (3–8 years) of these chemicals  and a strong stability over time observed in our pilot study, concentrations in the blood likely reflect relatively long-term PFAS exposures. Moreover, unlike many other persistent organic pollutants, PFASs are not lipophilic, and blood concentrations are therefore not affected by changes in the size of the lipid compartment . Third, we did not measure ghrelin, an orexigenic hormone regulating appetite, RMR, and other key physiological processes related to weight changes , and the interrelationship between PFASs and ghrelin during weight changes needs to be elucidated. Fourth, we did not apply Bonferroni correction in the analyses given the inter-correlation between the PFASs (rs ranged from 0.4 to 0.9), and the role of multiple testing could not be entirely excluded. Fifth, physical activity was assessed using the Baecke questionnaire, which might be subject to measurement errors, although a validation study conducted in US adults has shown reasonable validity of this questionnaire . In addition, although some covariates including education, smoking status, and physical activity were adjusted for in our study, we could not entirely exclude the possibility that unmeasured or residual confounding by socioeconomic and psychosocial factors, as well as participants’ usual diet, might partially account for the associations we observed. One particular concern is that PFASs are extensively used in food packaging due to their oil- and water-repellant characteristics . If some participants relapsed to their usual pre-randomization diet and this diet was rich in foods that are contaminated by PFASs through food packaging and are also dense in energy, they might thus have gained weight faster. However, when we further controlled for the frequency of craving hamburgers, French fries, or donuts at baseline assessed using a questionnaire, the results were largely unchanged. In addition, humans are exposed to PFASs through multiple pathways, including drinking water and contaminated seafood , although these factors are not established risk factors for weight gain. Moreover, we adjusted for the number of study sessions that participants attended, which is a measurement of compliance to the prescribed diet. Finally, lipophilic persistent pollutants with obesogenic effects (such as hexachlorobenzene [HCB] and dichlorodiphenyldichloroethylene [DDE]) might have confounded the associations of PFASs with changes in body weight and RMR. However, in 793 women participating in the Nurses’ Health Study II, weak associations were observed between PFASs and lipophilic persistent pollutants (e.g., the rs of PFOA and PFOS with HCB was 0.07 and 0.06, respectively, and the rs of PFOA and PFOS with DDE was 0.05 and 0.06, respectively), suggesting that confounding by these pollutants would not be substantial.
This basically screws up your health and immune system in the long run to be sure. Hope the UFC organizers change the rules so that they have a weigh in just prior to the fight. This will stop all this weight loss manipulations mania and will ensure fairness in the system, so that desperate fighters willing to risk their long term health don’t get an edge over the normal guys who want to have a longer healthier life after all these fights are done.
Before you even step foot on The Ranch in Malibu, you'll receive a kit in the mail full of daily and weekly goals to prepare you for the seven-day experience. Once there, it's time to power off the cell phones—there's no WiFi connection or cell service so you can focus on being present rather than finding the perfect Insta snap. With a group of 15 others, you'll push through four to five hours of group hiking, core work, yoga, and weight routines every day—none of which are optional—before recovering with a private afternoon massage (trust, your muscles are gonna need it) and daily nap (🙌 🙌). When there's downtime, sign up for a chiropractic, self-defense, or guided imagery session. Round it out with plenty of healthy eats and the occasional soak in the pool and you just might leave feeling reenergized to knock out those new year's resolutions you set oh-so-long ago.
Slimming Studios offers an array of enhanced weight loss products and services at centers across North Texas, and through trusted medical partners. Slimming Studios provides medical & noninvasive weight loss procedures, dietary supplements, weight control snacks, diet & workout plans, and weight loss counseling. Its purpose is to offer clients the very best in enhanced weight loss so they don’t have to wait to lose weight.
Good question, Matt. Quite frankly, for the athletes I work with it’s not an issue. Because they’re able to regain almost 100% of their strength, power, and endurance come fight night. So they have two advantages: 1) they’re at 100% in terms of performance capacity while their opponents, who probably cut weight in a less effective way, are likely only at 80%, 2) they’re also heavier (which grants strength leverage advantages). You can see there are a lot of variables here, though. And that’s why a scientific approach is warranted.
Whether or not you’re specifically aiming to cut carbs, most of us consume unhealthy amounts of sugar and refined carbohydrates such as white bread, pizza dough, pasta, pastries, white flour, white rice, and sweetened breakfast cereals. Replacing refined carbs with their whole-grain counterparts and eliminating candy and desserts is only part of the solution, though. Sugar is hidden in foods as diverse as canned soups and vegetables, pasta sauce, margarine, and many reduced fat foods. Since your body gets all it needs from sugar naturally occurring in food, all this added sugar amounts to nothing but a lot of empty calories and unhealthy spikes in your blood glucose.
A new German study found that when you drink 17 ounces of water (about two glasses) within a certain time frame, your metabolic rate shoots up by about 30 percent. Using these results, they estimate that by increasing your current water intake by 1.5 liters a day, a person would burn an extra 17,400 calories a year, resulting in about a five-pound weight loss.
Once you start to feel the effects of all these positive benefits, the result will be a healthier lifestyle you can stick to for the long term. “Many people tell me that when they feel happier, they want to sustain that feeling, so it just makes sense to choose habits that support it, like reaching for water instead of soda, taking the stairs rather than the elevator, and snacking on fruit and almonds in place of cookies,” Sass says. “External thoughts, like ‘I should do that,’ become internally driven choices that just feel right.” Focusing on the positive changes to your health rather than the number on the scale, Sass says, will likely make you feel more excited about other healthy behaviors, like drinking more water and eating more veggies. Make these 50 easy ways to lose weight naturally part of your routine.
My size has always been a part of my story. I never felt ashamed of my weight or pressured to change myself. I loved my body and loved eating good food—I was even a food blogger in New York City. Yet after 14 years in New York, my life turned upside down. I'd just ended a long-term relationship and needed something new, so I decided to make a big change and moved to Greenville, South Carolina.
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Break out the lemon wedges: Regular fish eaters tend to have lower levels of the hormone leptin — good because high levels of leptin have been linked to low metabolism and obesity, says Louis Aronne, M.D., an obesity specialist at the New York Presbyterian Weill Cornell Medical Center. Try to consume three to four servings of a fatty fish, such as salmon, tuna or mackerel, each week.
In a small German study, subjects who drank 16 ounces of water at a time experienced a 30 percent increase in metabolic rate during the following hour, burning an extra 24 calories. The researchers recommend cool water because the body expends extra calories warming it up to your body temperature. Water's not the only healthy drink. We sipped our way through dozens of smoothies, teas, and juices to find this list of the most delicious and nutritious liquids.
To maximize weight loss, guests will eat a high-protein/low-carb Paleolithic-inspired diet, starting the day with a fresh juice and continuing with alkalizing raw fruit and vegetables and fresh seafood. The trip fee includes an in-depth personal training course in the guest's home country, three weeks in advance in order to prepare for the trip, and one week on return.
It's an oldie but a goodie. The National Heart, Lung, and Blood Institute says that if you want to lose weight, the best way to make sure you stick with it is to make a few goals to work towards at the very beginning that aren't just about weight loss. Whether this is simply wanting to hit your five a day fruit and vegetables count or doing 30 minutes of exercise a day, they say this is the most effective way of beginning and maintaining weight loss.
No, genistein isn’t a trendy food item that’s about to blow up—it’s a compound that can help you lose weight. According to a study of female mice printed in The Journal of Nutrition, genistein has the power to decrease food intake and body weight. Scientists suspect this is because of the compound’s ability to turn down the genes for obesity and reduce your body’s capacity to store fat. To add some genistein to your diet, incorporate peanuts, beans, and lentils into your meals.
Good question…they actually do…and it’s a horrible idea! During the hours leading up to a fight, while an athlete is depleting water and glycogen, exercise should be kept to a minimum. Not only does the athlete need to recover from a hard training camp (thus, taper off exercise) so they can perform during their fight, they need to prevent excess stress. Cutting weight is pretty stressful as it is.
These simple guidelines outlined by our founder are still the powerful basic elements of our Integrated Healthy Lifestyle Model for women. Through the expertise of our highly trained professional staff and the thought leadership they bring to Green Mountain at Fox Run, our programs are always informed by the latest science and innovation. Based on the principles of mindfulness, our philosophy represents a triumph of fact over fiction, and of freedom over fear.
weight loss at 45 years old
My days here are very different than what they were before I came to the retreat. Most days I’m up at 8 a.m., but the last few weeks I’ve been inspired to wake up earlier to take a sunrise beach walk. I am not a morning person normally, but it’s turned out to be a surprisingly beautiful way to start the day. After that, I have breakfast. I can choose from a lot of different options as long as I stay withing my calorie goal for each meal. My favorite is either oatmeal and berries with coffee or scrambled eggs with vegetables. Sometimes I'll have a smoothie.
Carb crazy? Consider this: Refined carbohydrates, such as bread, potatoes and rice, create a surge in insulin that in turn drives down your resting metabolic rate, explains Aronne. "It's important to keep carbohydrates in your diet, but really focus on fruits, vegetables and whole grains, which have less of an effect on insulin levels," he explains. And when buying whole-grain breads and cereals, make sure the first ingredient listed is whole wheat, whole oat or cracked wheat.
Just because you’re trying to slim down, that doesn’t mean you have to forgo the occasional dessert splurge. There’s a simple solution to having your cake and eating it, too: Eat healthfully 80 percent of the time and reserve the remaining 20 percent of the time to cheat meals. Balance is key to sticking to your diet and dropping weight and maintaining it in the long run.
Are you considering a visit to a weight loss resort? Many smart dieters begin their journey away from home to get a clean break from the habits and lifestyle practices that led to weight gain in the first place. It's a smart approach if you can afford the luxury, but a visit to a health spa is not cheap, so it's smart to do your homework before you decide where to go.
Getting clear about why you want to lose weight will provide you with the fuel to keep going when your resolve starts to weaken (as—let’s be honest—it inevitably will). “I’d encourage those who are thinking of getting healthier to take some time to reflect and journal about what matters most,” Shirley Mast, R.N., B.S.N., and Take Shape For Life Health Coach, tells SELF.
We’ve already extolled the benefits of green and black teas, but they aren’t the only brews that can help you get slim; research indicates goji tea is another winner. Lycium barbarum, the plant from which goji berries are harvested, boasts a slimming effect. In a study published in the Journal of the American College of Nutrition, participants were either given a single dose of L. barbarum or a placebo after a meal. The researchers found that one hour after the dose, the goji group was burning calories at a rate 10 percent higher than the placebo group, and the effects lasted up to four hours. What’s more? Most goji teas are mixed with green tea, making the beverage a weight loss double whammy.
Considering that only 1 in 10 Americans meet their produce requirements, it’s pretty safe to say you need to eat more veggies. And no matter what food philosophy you subscribe to, veggies are a big part of the program. Vegetables have a lot going for them: They fill you up for very few calories, and they flood your body with the nutrients it needs to fight diseases, like heart disease, type 2 diabetes, and some cancers.
Make sure that everything you're eating is whole — as in nothing processed or packaged. Since salt is a preservative, these are the foods that are highest in sodium — something to keep in mind when planning your meals. Plan on making sure that all items you choose are fresh. That means filling up on fresh fruits and veggies, whole grains, low-fat dairy, and lean protein.
As far as grains go, quinoa is a great one to have around if you’re looking to lose weight. It’s packed with protein and fiber, and contains approximately 220 calories per cup, cooked. What’s more? Quinoa is one of the few plant foods that offer a complete set of amino acids, meaning it can be converted directly into muscle by the body. It’s also incredibly versatile, and can be eaten as part of a salad, tossed in a smoothie, or on its own as a side dish.
Stavrou, S., Nicolaides, N. C., Papageorgiou, I., Papadopoulou, P., Terzioglou, E., Chrousos, G. P., … Charmandari, E. (2016, July 31). The effectiveness of a stress-management intervention program in the management of overweight and obesity in childhood and adolescence. Journal of Molecular Biochemistry, 5(2), 63–70. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996635/
Berries are more than just morsels of sweetness that you can toss on yogurt or work into a smoothie; they can help you lose weight, too! Raspberries pack more fiber and liquid than most other fruits, which boosts satiety. They’re a rich source of ketones, antioxidants that can make you slimmer by incinerating stored fat cells. And like other berries, raspberries are loaded with polyphenols, powerful natural chemicals that have been shown to decrease the formation of fat cells and eliminate abdominal fat. Not to be outdone, research suggests blueberries can also help blast away stubborn belly fat by engaging your get-lean genes. After a 90-day trial, University of Michigan researchers discovered rats that were fed a blueberry-enriched diet showed significantly reduced belly fat compared to those who skipped the berries.
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During the weight-loss period, after multivariate adjustment including baseline levels of each metabolic parameter, plasma concentrations of PFOS, PFNA, and PFDA were inversely associated with changes in visceral fat mass (rs ranged from −0.19 to −0.27, all P < 0.05), and baseline PFOA was inversely associated with changes in HDL cholesterol (rs = −0.12, P < 0.01) (S5 Table). During the weight regain period, baseline PFOS, PFNA, and PFDA levels were positively associated with changes in some of the parameters, including waist circumference, insulin, and leptin (rs ranged from 0.10 to 0.15, all P < 0.05), and baseline PFOA and PFHxS were associated with a greater increase in visceral fat mass (rs = 0.30 and 0.27, respectively; both P < 0.05) (S5 Table). The results were largely similar when analyses were stratified by sex. In sensitivity analyses, the results did not materially change when further adjusting for study location (Boston or Baton Rouge) or participant compliance (number of sessions participants attended). The table in S1 Text shows the associations of baseline PFASs with gene expression in adipose tissue.
Fermented foods: These enhance the function of good bacteria while inhibiting the growth of bad bacteria. Sauerkraut, kimchi, kefir, yogurt, tempeh, and miso all contain good amounts of probiotics, which help to increase good bacteria. Researchers have studied kimchi widely, and study results suggest that it has anti-obesity effects. Similarly, studies have shown that kefir may help to promote weight loss in overweight women.
Part 1 — this post — details exactly how top fighters like Georges St. Pierre rapidly lose 20-30 pounds for “weigh-ins.” To refine the method, Nate performed this on himself, losing 20 pounds in 5 days. The unique part: Dr. Berardi and team measured key variables throughout the entire process, including the last “rehydration” phase. As Berardi put it:
And speaking of eating full-fat fare, a cutting-edge review published in PLOS One discovered that when it comes to reducing cardiovascular risk and promoting rapid weight loss, low-carb diets are superior to low-fat diets. Can’t imagine fully committing to a low-carb lifestyle? Start by eliminating empty sources of carbs from your diet such as white bread, desserts, and sugary drinks.
Delight in our first-rate spa. Nutrition and fitness are just part of your holistic journey. Retreat to The Indigo Spa to rejuvenate your body and soul with spa services designed to complement healthy lifestyles. Nurture yourself with natural treatments that inspire peace and tranquility, and reward yourself for your progress in Hilton Head Health’s weight loss and wellness programs.
That’s one way the beautifully presented, animal-protein-centric food comes in. Of course, protein is excellent fuel, but the meal plan is also meant to prove that healthy choices don’t require deprivation. You can eat more or less like a normal American, red meat and all (though they accommodate vegetarians handily). Between the reasonably sized meals and copious snacks (to keep blood sugar even), I was never hungry, and occasionally doubted that it really added up to 1,200 calories a day (1,400 for men)—though the fat-measuring scale convinced many of us otherwise.
(CONFRONT YOUR FEARS) A friend reminded me of this recently and as he did I remembered a fear. It was fresh and still made my skin prickle with anticipation even though it started many years ago. It feels like yesterday and I’m sure you have those moments too, the ones that never left you even though you shoved them away for a long time. It starts with the 6th grade physical fitness tests. They were the worst things ever except for the talented few that got the bright blue patches. These tests traumatized me. Mostly, the timed mile. Even though we were children it was expected that we run it and everyone did- except me. There wasn't much kindness or compassion in the process and once I realized that, it was something that I dreaded, avoided and ultimately feared. It’s so easy to take ourselves out of even trying when there’s no encouragement to try. When your gym clothes don’t fit right and you’re afraid of the attention that coming in last place will bring you. Year after year this test happened and year after year I walked it & then cried, faked being sick and then ultimately stopped showing up. I’ve never run a mile without stopping and it’s always been on my list of things I want to do but didn’t know if I would. But I want to now. No matter how long it takes me and for no one else other than myself. And now, after being here I would rather try and fail than not try at all. Now’s the time to tackle the fears, all of them no matter how small or how big. So one day I started by asking my trainer if we could work on learning how to run in between strength training. My voice raised and I stumbled over my words and I felt self-conscious again like that chubby little 6th grader in too tight of gym shorts. I was nervous putting this out into the world. He said absolutely and then the next day we did. Just like that. He took me through some movements to work on my stride and made me feel like this was something that I could do. And step by step it was. Only 45 seconds this time, but 45 seconds closer to my goal and each step we take towards our goals away from our fears count. Not always the end result matters most, but the time it takes to jog there.
The scientists ferried 20 overweight, middle-aged men by train and cable car to a research station perched 1,000 feet below the peak of Germany's highest mountain, Zugspitze. During the week-long stay, the men could eat and drink as much as they liked and were forbidden from any exercise other than leisurely strolls. The team measured the men's weight, metabolic rate, levels of hunger and satiety hormones before, during, and after their mountain retreat After a week up high, the subjects lost an average of 3 pounds. A month later, they were still 2 pounds lighter. The scientists' data showed this was likely because they ate about 730 calories less at high altitudes than they did at normal elevations. They may have felt less hungry, in part, because levels of leptin, the satiety hormone, surged during the stay, while grehlin, the hunger hormone, remained unchanged. Their metabolic rate also spiked, meaning they burned more calories than they usually did. A high-altitude weight loss strategy could be viable, though studies have shown peoples' appetites bounce back after about six months at high elevation, Leissner said. “If you could do intermittent periods for one week, then go down, and then go back up, this might actually be helpful.”
For an absolutely breathtakingly beautiful life-changing experience, head to Akasha. Their retreat center is set in Romania, which is an off-the-beaten-path destination. Spend your days exploring the countryside with mountain treks and yoga on the summits. Then go back home and tell everyone about how wonderful this beautiful center AND Romania was.
Lately, we’re noticing protein-packed everything—from breads to nut butter and milk. While you don’t need to load up on weird franken-foods to amp up your intake of the nutrient, if you’re trying to drop a few pounds, then it’s wise to keep some high-protein snacks on hand. Noshing on these can prevent eating something high-calorie every time hunger strikes.
According to the American Heart Association, losing as little as five to ten pounds in overweight people may help lower blood pressure. Research clearly supports this link. With extra weight, your body has to work harder to pump blood, which can damage blood vessels and harden the arteries. “Being obese also increases the body’s demand for oxygen and increases blood volume,” says Ali Webster, PhD, RD, associate director of nutrition communications for the International Food Information Council Foundation. Try these 42 fast, easy tips to lose weight.
Plasma concentrations of PFOS, PFOA, perfluorononanoic acid (PFNA), perfluorohexanesulfonic acid (PFHxS), and perfluorodecanoic acid (PFDA) were measured at baseline only, using a sensitive and reliable method based on online solid phase extraction and liquid chromatography coupled to a triple quadropole mass spectrometer , with minor modifications. Due to the long elimination half-lives of the PFASs and incomplete samplings, we did not measure plasma PFAS levels during the trial. For all major PFASs, the concentrations were above the limit of detection (0.05 ng/ml), and the inter- and intra-assay coefficients of variation were <6.3% and <6.1%, respectively.